T cell formation

A genome wide screen of 185 G3 pedigrees of ENU mutagenized C57BL/6 mice for defects in T and B cell production by members of Prof Chris Goodnow.s laboratory yielded a heritable mutant in every 12th pedigree. Each mutant has been mapped and the causal mutation identified by sequencing cDNAs encoded within the mapped interval. Because there is no ...

Immunity and Tolerance

A series of immunization and autoantibody tests of serum was developed and used by Dr Carola Vinuesa and her team at the John Curtin School to screen two libraries of ~200 G3 pedigrees of mutagenized B6 mice intercrossed with B10.BR. Approximately every seventh pedigree yielded a true-breeding variant strain with spontaneous production of ...

Deafness

A simple test for hearing, by observing the freeze-reflex in response to a human inaudible click, was used by members of Dr Henrik Dahl's laboratory at the Murdoch Children.s Research Institute initially to screen ~40 pedigrees of G3 mice. Three true-breeding deaf strains were identified, and are now being analysed and the mutations mapped. Based on this data, an NHMRC project ...

Male Fertility

After other screens were completed, members of Dr Moira O.Bryan's laboratory at the Monash Institute of Reproduction and Development euthanased G3 males from ~100 pedigrees, performed sperm counts and cryopreserved the sperm, and analysed spermatid differentiation by histological analysis of the testes. Approximately 10 variant strains were identified, with three ...