Mapping/Linkage Analysis

APF Genomics Research services provides space, equipment and expertise to run small or large projects to map the chromosomal location and aid in identification of the affected gene of new gene-variant mouse strains obtained from the ENU gene-variant Libraries, the Phenome Bank, spontaneous mutations and causative polymorphisms between two inbred strains. Our service offers a number of genome screens for mapping using a fluorescent Amplifluor SNV technology (Chemicon). Options include a proximal/distal screen with markers at each end of the chromosomes plus one central to the longer chromosomes and genome wide (10-20Mb spacing) marker panels for various mapping crosses. For fine mapping projects we have a continually expanding set of additional markers and in addition will custom design assays to SNVs in your mapped intervals.

To complete the mapping service the Genomics team offers resequencing of exons within a mapped interval. We have the ability to efficiently design primers for resequencing candidate genes or all exons within a defined interval. The sequencing assays are run at the AGRF, Brisbane and then expertly analysed at the APF to locate the causal mutation.

Follow this link to proceed to the Ordering page to submit samples for mapping/linkage analysis